Printing and Erasing of DNA?Based Photoresists Inside Synthetic Cells

In the pursuit of producing functioning synthetic cells from bottom-up, DNA nanotechnology has proven to be a powerful tool. However, crowded yet highly organized arrangement in living cells, bridging nano- micron-scale, remains challenging recreate with DNA-based architectures. Here, laser microprinting is established print and erase shape-controlled hydrogels inside confinement water-in-oil droplets giant unilamellar lipid vesicles (GUVs). The photoresist consists photocleavable inactive linker, which interconnects Y-shaped motifs when activated by local irradiation 405 nm laser. An alternative linker design allows custom features preformed hydrogel feature sizes down 1.38 ?m. present work demonstrates that can serve as an internal support stabilize non-spherical GUV shapes. Overall, photoresists for printing allow build up architectures on interior light, diversifies toolbox bottom-up biology.